Association of taste receptor gene polymorphisms with dental caries

Abstract This study was performed to evaluate the interplay between dental caries, nutritional status, and genetic polymorphisms in TAS1R1 and TAS1R2 (taste receptor, type 1, member 1 and 2) in preschool children and pre-adolescents. We included 525 subjects (306 preschool children and 219 pre-adolescents). Parents/caregivers answered a self-administered questionnaire about their children's systemic health, characteristics, oral hygiene habits, and diet. Clinical examination was performed to evaluate dental caries and nutritional status. Saliva samples were collected for DNA extraction. The genotyping of rs17492553 ( TAS1R1 ), rs3935570, and rs4920566 ( TAS1R2 ) polymorphisms was performed using real-time PCR with Taqman Genotyping Master Mix and SNP assay. Both univariate and multivariate Poisson regression analyses with robust variance were used for the data analysis. In preschool children, consumption of sweets between meals increased the prevalence of dental caries by 85% (PR c = 1.85; 95%CI 1.39-2.46; p < 0.001), whereas in pre-adolescents, this prevalence increased by 34% (PR a = 1.34; 95%CI 1.11-1.62; p = 0.002), regardless of genetic polymorphisms . Moreover, individuals carrying at least one allele C in rs17492553 presented 23% more prevalence of dental caries (PR a = 1.23; 95%CI 1.02-1.49 p = 0.030). Nutritional status was not associated with dental caries, neither with genetic polymorphisms . Consumption of sweets between meals increased the prevalence of dental caries. In pre-adolescents, rs17492553 genetic polymorphism in TAS1R1 was associated with dental caries.

Oral health-related quality of life of 8-10 year-old children with traumatic dental injury

Introduction: Children presents high prevalence of traumatic dental injury (TDI). Objective: It is very important to detect the impact on oral health related to quality of life (OHRQoL) in this age group. Methods: Based on this, a cross sectional study was carried out using a convenience sample. Children from 8-10 years with TDI in permanent teeth were recruited over an 18 month period, from a public University. Data about OHRQoL using the Brazilian version of CPQ 8-10 (B-CPQ 8- 0 ) were collected. TDI were classified according to Andreasen criteria and grouped according to its severity. Variations in the scores and impact of TDI on OHRQoL according to age, gender and TDI severity were examined using nonparametric statistical tests (Kruskall-Wallis and Mann-Whitney test, p<0.05). Results: From a sample of 255 children, 41 healthy children that suffered TDI in permanent were included in the study. The mean B-CPQ 8-10 scores were 29.7 (SD 14.3). Emotional and social wellbeing domais had the highest negative score impact average 8.8 (SD 5.9) and 9.8 (SD 6.3), respectively. Gender or TDI severity did not present relation to impact on OHRQoL. In relation to age, 10-year-old children had more impact on their OHRQoL on the overall scale (p<0.05) and for the functional limitation subscale (p<0.05). Conclusion: TDI impacts negatively on OHRQoL of children aged from 8-10 years old.

Introdução: Crianças apresentam alta prevalência de traumatismo dentário (TD).Objetivo: Detectar o impacto do TD na qualidade de vida relacionada à saúde bucal (QVRSB) nessa faixa etária. Métodos: Um estudo transversal foi realizado com amostra de conveniência recrutando-se crianças de 8-10 anos com TD em dente permanente por um período de 18 meses em clínica pública. Dados sobre a QVRSB foram coletados através da versão brasileira do CPQ 8-10 (B-CPQ 8-10 ). O TD foi classificado de acordo com os critérios de Andreasen e agrupado de acordo com sua gravidade. As variações nos escores e o impacto do TD na QVRSB, de acordo com idade, gênero e gravidade do TD foram examinados por meio de testes estatísticos não paramétricos (teste de Kruskall-Wallis e Mann-Whitney, p <0,05). Resultados: De uma amostra de 255 crianças, 41 crianças saudáveis que sofreram TD em dentes permanentes foram incluídas no estudo. O escore médio do B-CPQ 8-10 foi 29,7 (DP 14,3). Os domínios bem-estar emocional e social apresentaram maior impacto negativo com média de 8,8 (DP 5,9) e 9,8 (DP 6,3), respectivamente. A diferença de gênero e a gravidade do TD não apresentaram relação de impacto na QVRSB. Em relação a idade, crianças de 10 anos tiveram maior impacto na escala global da QVRSB (p <0,05) e na subescala de limitação funcional (p <0,05). Conclusão: O TD impacta negativamente a QVRSB de crianças de 8 a 10 anos de idade.